Active Recombinant Human SOST protein, His-tagged, Biotinylated

• Cat.No.: SOST-270H
• Product Overview: Biotinylated Recombinant Human SOST protein(Gln 24 - Tyr 213), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln 24 - Tyr 213
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human LRP-6 (20-630), Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human SOST, His Tag, primary amine labeling with a linear range of 0.005-0.313 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 22.3 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SOST sclerostin [ Homo sapiens ]
• Official Symbol: SOST
• Synonyms: SOST; sclerostin; sclerosteosis; VBCH
• Gene ID: 50964
• mRNA Refseq: NM_025237
• Protein Refseq: NP_079513
• MIM: 605740
• UniProt ID: Q9BQB4