Recombinant Mouse Sod2, His-tagged

• Cat.No.: Sod2-56M
• Product Overview: Recombinant mouse Sod2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Sod2 also known as superoxide dismutase [Mn], mitochondrial, is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial matrix protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this protein have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.
• Source: E.coli
• Species: Mouse
• Tag: His
• Form: Liquid, In Phosphate buffered saline (pH7.4) containing 10% glycerol, 1mM DTT
• Molecular Mass: 24.6kDa (221aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSKHSLPDL PYDYGALEPH INAQIMQLHH SKHHAAYVNN LNATEEKYHE ALAKGDVTTQ VALQPALKFN GGGHINHTIF WTNLSPKGGG EPKGELLEAI KRDFGSFEKF KEKLTAVSVG VQGSGWGWLG FNKEQGRLQI AACSNQDPLQ GTTGLIPLLG IDVWEHAYYL QYKNVRPDYL KAIWNVINWE NVTERYTACK K
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: Sod2 superoxide dismutase 2, mitochondrial [ Mus musculus ]
• Official Symbol: Sod2
• Synonyms: SOD2; superoxide dismutase 2, mitochondrial; superoxide dismutase [Mn], mitochondrial; manganese SOD; manganese superoxide dismutase; MnSOD; Sod-2; MGC6144
• Gene ID: 20656
• mRNA Refseq: NM_013671
• Protein Refseq: NP_038699
• Pathway: Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem; One carbon metabolism and related pathways, organism-specific biosystem; Oxidative Stress, organism-specific biosystem; Peroxisome, organism-specific biosystem; Peroxisome, conserved biosystem; superoxide radicals degradation, organism-specific biosystem
• Function: DNA binding; identical protein binding; manganese ion binding; metal ion binding; oxidoreductase activity; oxygen binding; superoxide dismutase activity; superoxide dismutase activity; superoxide dismutase activity

Reviews

01/10/2021

It has earned my trust with its outstanding stability and reproducibility.

10/04/2016

After using this protein reagent, my protein gel electrophoresis results are notably clear, aiding in analysis and interpretation.

07/30/2016

Engaging confidently in academic discussions and collaborations with peers is possible due to this reagent's exceptional performance.

Q&As

What are the current research directions regarding SOD2?

Current research on SOD2 focuses on understanding its role in different disease contexts and identifying potential therapeutic targets. Investigations are underway to elucidate the molecular mechanisms underlying SOD2 regulation, its involvement in age-related diseases, and the development of novel strategies to enhance SOD2 activity for therapeutic purposes. Additionally, studies are exploring the interplay between SOD2 and other cellular processes, aiming to uncover new insights into its biological functions.

What therapeutic strategies target SOD2 for disease treatment?

Several approaches have been explored to enhance SOD2 activity as a potential therapeutic strategy. These include the use of small molecule activators, such as resveratrol and melatonin, which can upregulate SOD2 expression and improve cellular antioxidant defense. Additionally, gene therapy approaches aiming to increase SOD2 levels or activity have shown promise in preclinical studies.

How does SOD2 contribute to cellular senescence and aging?

As cells age, the expression and activity of SOD2 decline, resulting in increased levels of reactive oxygen species (ROS) and oxidative damage. The accumulation of ROS can promote cellular senescence, a state of irreversible growth arrest. SOD2 plays a crucial role in mitigating ROS-mediated DNA damage and preserving cellular integrity, thus influencing the aging process.

How is the expression of SOD2 regulated?

The expression of SOD2 is regulated by various transcription factors, including nuclear factor erythroid 2-related factor 2 (NRF2) and forkhead box O3 (FOXO3). These transcription factors bind to the promoter region of the SOD2 gene and initiate its transcription. Additionally, post-translational modifications, such as acetylation and phosphorylation, can also modulate the activity and stability of SOD2.

What is the significance of SOD2 polymorphisms in disease susceptibility?

Certain genetic variations or polymorphisms in the SOD2 gene have been associated with altered enzyme activity and increased susceptibility to certain diseases, including neurodegenerative disorders, cardiovascular diseases, and cancer. These polymorphisms can affect the antioxidant capacity of SOD2, leading to an imbalance between reactive oxygen species generation and scavenging, thereby contributing to disease pathogenesis.

What is the primary function of SOD2 in cellular physiology?

SOD2, also known as mitochondrial superoxide dismutase, is an enzyme responsible for the dismutation of superoxide radicals into hydrogen peroxide and molecular oxygen within the mitochondria. This antioxidant activity helps to protect cells from oxidative stress and maintain redox homeostasis in the mitochondria.

How does SOD2 interact with other proteins and signaling pathways?

SOD2 is known to interact with various proteins and signaling pathways to regulate cellular processes. For instance, SOD2 interacts with the nuclear factor kappa B (NF-κB) pathway, modulating its activity and downstream gene expression. SOD2 also interacts with other antioxidant enzymes, such as catalase and glutathione peroxidase, forming a coordinated antioxidant defense system within cells.