Recombinant Mouse Smurf2 Protein, Myc/DDK-tagged
Cat.No. : | Smurf2-5985M |
Product Overview : | Purified recombinant protein of mouse full-length SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), with C-terminal MYC/DDK tag, expressed in HEK293T cells. |
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Species : | Mouse |
Source : | HEK293 |
Tag : | DDK&Myc |
Description : | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation. |
Molecular Mass : | 86.2 kDa |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >50 μg/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name | Smurf2 SMAD specific E3 ubiquitin protein ligase 2 [ Mus musculus (house mouse) ] |
Official Symbol | Smurf2 |
Synonyms | SMURF2; SMAD specific E3 ubiquitin protein ligase 2; E3 ubiquitin-protein ligase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; AI558114; AI649275; 2810411E22Rik |
Gene ID | 66313 |
mRNA Refseq | NM_025481 |
Protein Refseq | NP_079757 |
UniProt ID | A2A5Z6 |
◆ Recombinant Proteins | ||
SMURF2-4835H | Recombinant Human SMURF2 protein, His-tagged | +Inquiry |
SMURF2-8505M | Recombinant Mouse SMURF2 Protein, His (Fc)-Avi-tagged | +Inquiry |
SMURF2-6400Z | Recombinant Zebrafish SMURF2 | +Inquiry |
SMURF2-6287H | Recombinant Human SMURF2 Protein (His246-Glu400), N-His tagged | +Inquiry |
SMURF2-2827H | Recombinant Human SMURF2, His-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
SMURF2-1646HCL | Recombinant Human SMURF2 293 Cell Lysate | +Inquiry |
Not For Human Consumption!
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