Recombinant Mouse Smurf2 Protein, Myc/DDK-tagged

Cat.No. : Smurf2-5985M
Product Overview : Purified recombinant protein of mouse full-length SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
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Species : Mouse
Source : HEK293
Tag : DDK&Myc
Description : E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation.
Molecular Mass : 86.2 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name Smurf2 SMAD specific E3 ubiquitin protein ligase 2 [ Mus musculus (house mouse) ]
Official Symbol Smurf2
Synonyms SMURF2; SMAD specific E3 ubiquitin protein ligase 2; E3 ubiquitin-protein ligase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; AI558114; AI649275; 2810411E22Rik
Gene ID 66313
mRNA Refseq NM_025481
Protein Refseq NP_079757
UniProt ID A2A5Z6

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