Recombinant Human SMURF2 Protein, Myc/DDK-tagged, C13 and N15-labeled
Cat.No. : | SMURF2-5351H |
Product Overview : | SMURF2 MS Standard C13 and N15-labeled recombinant protein (NP_073576) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
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Species : | Human |
Source : | HEK293 |
Tag : | DDK&Myc |
Description : | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. |
Molecular Mass : | 86.2 kDa |
AA Sequence : | MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVETRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : | Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : | 50 μg/mL as determined by BCA |
Storage Buffer : | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
Gene Name | SMURF2 SMAD specific E3 ubiquitin protein ligase 2 [ Homo sapiens (human) ] |
Official Symbol | SMURF2 |
Synonyms | SMURF2; SMAD specific E3 ubiquitin protein ligase 2; E3 ubiquitin-protein ligase SMURF2; hSMURF2; E3 ubiquitin ligase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; MGC138150; DKFZp686F0270; |
Gene ID | 64750 |
mRNA Refseq | NM_022739 |
Protein Refseq | NP_073576 |
MIM | 605532 |
UniProt ID | Q9HAU4 |
◆ Recombinant Proteins | ||
SMURF2-4835H | Recombinant Human SMURF2 protein, His-tagged | +Inquiry |
SMURF2-6400Z | Recombinant Zebrafish SMURF2 | +Inquiry |
SMURF2-468H | Recombinant Human SMURF2, FLAG-tagged | +Inquiry |
SMURF2-138H | Recombinant Human SMURF2, GST-tagged | +Inquiry |
SMURF2-1714H | Recombinant Human SMURF2 Protein (1-748 aa), His-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
SMURF2-1646HCL | Recombinant Human SMURF2 293 Cell Lysate | +Inquiry |
Not For Human Consumption!
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