Recombinant Mouse Ruvbl1 Protein, Myc/DDK-tagged

• Cat.No.: Ruvbl1-5653M
• Product Overview: Purified recombinant protein of mouse full-length RuvB-like protein 1 (Ruvbl1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. Essential for cell proliferation. May be able to bind plasminogen at cell surface and enhance plasminogen activation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 50.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ruvbl1 RuvB-like protein 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ruvbl1
• Synonyms: RUVBL1; RuvB-like protein 1; ruvB-like 1; Pontin 52; DNA helicase p50; 49 kDa TBP-interacting protein; 49 kDa TATA box-binding protein-interacting protein; Tip49a; Pontin52; 2510009G06Rik
• Gene ID: 56505
• mRNA Refseq: NM_019685
• Protein Refseq: NP_062659
• UniProt ID: P60122