Recombinant Mouse Rfx3 Protein, Myc/DDK-tagged

• Cat.No.: Rfx3-5481M
• Product Overview: Purified recombinant protein of mouse full-length regulatory factor X, 3 (influences HLA class II expression) (Rfx3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5). Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells.
• Molecular Mass: 83.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rfx3 regulatory factor X, 3 (influences HLA class II expression) [ Mus musculus (house mouse) ]
• Official Symbol: Rfx3
• Synonyms: RFX3; regulatory factor X, 3 (influences HLA class II expression); transcription factor RFX3; regulatory factor X 3; MRFX3; C230093O12Rik
• Gene ID: 19726
• mRNA Refseq: NM_011265
• Protein Refseq: NP_035395
• UniProt ID: P48381