Recombinant Mouse Rapgef4 Protein, Myc/DDK-tagged

• Cat.No.: Rapgef4-5378M
• Product Overview: Purified recombinant protein of mouse full-length Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 113.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rapgef4 Rap guanine nucleotide exchange factor (GEF) 4 [ Mus musculus (house mouse) ]
• Official Symbol: Rapgef4
• Synonyms: RAPGEF4; Rap guanine nucleotide exchange factor (GEF) 4; rap guanine nucleotide exchange factor 4; cAMP-GEFII; exchange factor directly activated by cAMP 2; exchange protein directly activated by cAMP 2; cAMP-regulated guanine nucleotide exchange factor IIB; cAMP-dependent Rap1 guanine-nucleotide exchange factor; Epac2; EPAC 2; Epac-2; mKIAA4040; 1300003D15Rik; 5730402K07Rik; 6330581N18Rik; KIAA4040
• Gene ID: 56508
• mRNA Refseq: NM_019688
• Protein Refseq: NP_062662
• UniProt ID: Q9EQZ6