Recombinant Mouse Rad18 Protein, Myc/DDK-tagged

• Cat.No.: Rad18-5347M
• Product Overview: Purified recombinant protein of mouse full-length RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 54.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rad18 RAD18 E3 ubiquitin protein ligase [ Mus musculus (house mouse) ]
• Official Symbol: Rad18
• Synonyms: Rad18; RAD18 E3 ubiquitin protein ligase; Rad18sc; 2810024C04Rik; E3 ubiquitin-protein ligase RAD18; RAD18 homolog; RING-type E3 ubiquitin transferase RAD18; mRAD18Sc; post-replication repair protein RAD18SC; postreplication repair protein RAD18; EC 2.3.2.27
• Gene ID: 58186
• mRNA Refseq: NM_021385
• Protein Refseq: NP_067360
• UniProt ID: Q9QXK2