Recombinant Mouse Polk Protein, Myc/DDK-tagged

• Cat.No.: Polk-4991M
• Product Overview: Purified recombinant protein of mouse full-length polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
• Molecular Mass: 82.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Polk polymerase (DNA directed), kappa [ Mus musculus (house mouse) ]
• Official Symbol: Polk
• Synonyms: POLK; polymerase (DNA directed), kappa; DNA polymerase kappa; DINP; DINB protein; DinB homolog 1; DNA damage-inducible protein b; DNA-directed DNA polymerase kappa; Dinb1
• Gene ID: 27015
• mRNA Refseq: NM_012048
• Protein Refseq: NP_036178
• UniProt ID: Q9QUG2