Recombinant Human POLK, MYC/DDK-tagged

• Cat.No.: POLK-837H
• Product Overview: Recombinant Human POLK, fused with C-terminal MYC/DDK, was expressed in HEK293 Cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass.
• Molecular Mass: 98.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: POLK polymerase (DNA directed) kappa [ Homo sapiens (human) ]
• Official Symbol: POLK
• Synonyms: POLK; DINP; POLQ; DINB1; polymerase (DNA directed) kappa; DNA polymerase kappa; NP_057302.1; EC 2.7.7.7
• Gene ID: 51426
• mRNA Refseq: NM_016218
• Protein Refseq: NP_057302
• MIM: 605650
• UniProt ID: Q9UBT6
• Chromosome Location: 5q13
• Pathway: Fanconi anemia pathway
• Function: DNA-directed DNA polymerase activity; damaged DNA binding; metal ion binding