Recombinant Mouse Peli1 Protein, Myc/DDK-tagged

• Cat.No.: Peli1-4790M
• Product Overview: Purified recombinant protein of mouse full-length pellino 1 (Peli1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation. Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3. Negatively regulates necroptosis by reducing RIPK3 expression. Mediates 'Lys-63'-linked ubiquitination of RIPK1.
• Molecular Mass: 46.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Peli1 pellino 1 [ Mus musculus (house mouse) ]
• Official Symbol: Peli1
• Synonyms: Peli1; pellino 1; AA409794; AI586297; D11Ertd676; D11Ertd676e; 2810468L03Rik; A930031K15Rik; E3 ubiquitin-protein ligase pellino homolog 1; RING-type E3 ubiquitin transferase pellino homolog 1; protein pellino homolog 1; EC 2.3.2.27
• Gene ID: 67245
• mRNA Refseq: NM_023324
• Protein Refseq: NP_075813
• UniProt ID: Q8C669