Recombinant Mouse Parn Protein, Myc/DDK-tagged

• Cat.No.: Parn-4677M
• Product Overview: Purified recombinant protein of mouse full-length poly(A)-specific ribonuclease (deadenylation nuclease) (Parn), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. Also able to recognize poly(A) tails of microRNAs such as MIR21 and H/ACA box snoRNAs (small nucleolar RNAs) leading to leading to microRNAs degradation or snoRNA increased stability.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 71.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Parn poly(A)-specific ribonuclease (deadenylation nuclease) [ Mus musculus (house mouse) ]
• Official Symbol: Parn
• Synonyms: PARN; poly(A)-specific ribonuclease (deadenylation nuclease); poly(A)-specific ribonuclease PARN; polyadenylate-specific ribonuclease; DAN; 1200003I18Rik
• Gene ID: 74108
• mRNA Refseq: NM_028761
• Protein Refseq: NP_083037
• UniProt ID: Q8VDG3