Recombinant Mouse Notum Protein, Myc/DDK-tagged

• Cat.No.: Notum-1434M
• Product Overview: Purified recombinant protein of mouse full-length RIKEN cDNA 5730593N15 gene (cDNA clone MGC:37936 IMAGE:5125723), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
• Molecular Mass: 36.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Notum notum palmitoleoyl-protein carboxylesterase [ Mus musculus (house mouse) ]
• Official Symbol: Notum
• Synonyms: Notum; notum palmitoleoyl-protein carboxylesterase; 5730593N15Rik; palmitoleoyl-protein carboxylesterase NOTUM; notum pectinacetylesterase homolog; protein notum homolog; EC 3.1.1.98
• Gene ID: 77583
• mRNA Refseq: NM_175263
• Protein Refseq: NP_780472
• UniProt ID: Q8R116