Recombinant Mouse Med22 Protein, Myc/DDK-tagged

• Cat.No.: Med22-4015M
• Product Overview: Purified recombinant protein of mouse full-length mediator complex subunit 22 (Med22), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
• Molecular Mass: 16.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Med22 mediator complex subunit 22 [ Mus musculus (house mouse) ]
• Official Symbol: Med22
• Synonyms: MED22; mediator complex subunit 22; mediator of RNA polymerase II transcription subunit 22; surfeit gene 5; surfeit locus protein 5; Surf5; Surf-5; AW212655; AW558812
• Gene ID: 20933
• mRNA Refseq: NM_011513
• Protein Refseq: NP_035643
• UniProt ID: Q62276