Recombinant Mouse Lipe Protein, His-tagged

• Cat.No.: Lipe-1740M
• Product Overview: Recombinant Mouse Lipe Protein (Gln652-Asn759) with N-His tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Gln652-Asn759
• Description: Enables carboxylic ester hydrolase activity; protein kinase binding activity; and serine hydrolase activity. Involved in ether lipid metabolic process and lipid catabolic process. Acts upstream of or within cellular lipid catabolic process and cellular response to cold. Located in cytosol; lipid droplet; and membrane. Is expressed in adipose tissue; ileum; and male reproductive gland or organ. Human ortholog(s) of this gene implicated in arteriosclerosis; familial partial lipodystrophy type 6; and hypertension. Orthologous to human LIPE (lipase E, hormone sensitive type).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 15.6 kDa
  Accurate Molecular Mass: 16 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Lipe lipase, hormone sensitive [ Mus musculus (house mouse) ]
• Official Symbol: Lipe
• Synonyms: LIPE; lipase, hormone sensitive; hormone-sensitive lipase; HSL; 4933403G17Rik
• Gene ID: 16890
• mRNA Refseq: NM_001039507
• Protein Refseq: NP_001034596
• UniProt ID: P54310