Recombinant Human LIPE Protein, His-tagged

• Cat.No.: LIPE-2395H
• Product Overview: Recombinant Human LIPE Protein (Met302-Leu425) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met302-Leu425
• Description: The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 17.3 kDa
  Accurate Molecular Mass: 17 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: LIPE lipase, hormone-sensitive [ Homo sapiens (human) ]
• Official Symbol: LIPE
• Synonyms: LIPE; lipase, hormone-sensitive; hormone-sensitive lipase; HSL; hormone-sensitive lipase testicular isoform; LHS
• Gene ID: 3991
• mRNA Refseq: NM_005357
• Protein Refseq: NP_005348
• MIM: 151750
• UniProt ID: Q05469