Recombinant Mouse Kdm4a Protein, Myc/DDK-tagged

• Cat.No.: Kdm4a-3681M
• Product Overview: Purified recombinant protein of mouse full-length lysine (K)-specific demethylase 4A (Kdm4a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
• Molecular Mass: 120.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Kdm4a lysine (K)-specific demethylase 4A [ Mus musculus (house mouse) ]
• Official Symbol: Kdm4a
• Synonyms: KDM4A; lysine (K)-specific demethylase 4A; lysine-specific demethylase 4A; jumonji domain containing 2A; jumonji domain-containing protein 2A; jmjC domain-containing histone demethylation protein 3A; Jmjd2; JHDM3A; Jmjd2a; mKIAA0677; D4Ertd222e; MGC28562
• Gene ID: 230674
• mRNA Refseq: NM_001161823
• Protein Refseq: NP_001155295
• UniProt ID: Q8BW72