Recombinant Mouse Itk Protein, GST & His-tagged

• Cat.No.: Itk-861M
• Product Overview: Recombinant mouse ITK / EMT (Q03526) protein kinase domain (Arg 351-Leu 619) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Arg 351-Leu 619
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant mouse ITK /GST chimera consists of 506 amino acids and has a calculated molecular mass of 58.4 kDa. The recombinant protein migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Itk IL2 inducible T cell kinase [ Mus musculus ]
• Official Symbol: Itk
• Synonyms: ITK; IL2 inducible T cell kinase; tyrosine-protein kinase ITK/TSK; kinase EMT; kinase TLK; T-cell-specific kinase; IL2-inducible T-cell kinase; IL-2-inducible T-cell kinase; interleukin-2-inducible T cell kinase; Emt; Tsk; Tcsk
• Gene ID: 16428
• mRNA Refseq: NM_010583
• Protein Refseq: NP_034713