Active Recombinant Full Length Human ITK Protein, DDK-tagged, Biotinylated

• Cat.No.: ITK-1241HF
• Product Overview: Full-length human ITK [2-620(end) amino acids of accession number NP_005537.3] was expressed as N- terminal DYKDDDDK tagged, biotinylated protein (75 kDa) using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose and activated with ATP. Activated protein was purified by using gel filtration.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Flag
• Protein Length: 2-620(end)
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Purity: 90%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: IL2-inducible T-cell kinase
• Official Symbol: ITK
• Synonyms: ITK;EMT;LPFS1;LYK;PSCTK2
• Protein Refseq: NP_005537.3
• UniProt ID: Q08881