Recombinant Mouse Itgb3bp Protein, Myc/DDK-tagged

• Cat.No.: Itgb3bp-3607M
• Product Overview: Purified recombinant protein of mouse full-length integrin beta 3 binding protein (beta3-endonexin) (Itgb3bp), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Transcription coregulator that can have both coactivator and corepressor functions. Involved in the coactivation of nuclear receptors for retinoid X (RXRs) and thyroid hormone (TRs) in a ligand-dependent fashion. In contrast, it does not coactivate nuclear receptors for retinoic acid, vitamin D, progesterone receptor, nor glucocorticoid. Acts as a coactivator for estrogen receptor alpha. Acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. Induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. May also act as an inhibitor of cyclin A-associated kinase. Also acts a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex.
• Molecular Mass: 20.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Itgb3bp integrin beta 3 binding protein (beta3-endonexin) [ Mus musculus (house mouse) ]
• Official Symbol: Itgb3bp
• Synonyms: ITGB3BP; integrin beta 3 binding protein (beta3-endonexin); centromere protein R; CENP-R; beta3-endonexin; nuclear receptor-interacting factor 3; AU022583; 4930471O16Rik
• Gene ID: 67733
• mRNA Refseq: NM_026348
• Protein Refseq: NP_080624
• UniProt ID: Q9CQ82