Recombinant Mouse Gzmk protein(Gln44~Val227), His-tagged

• Cat.No.: Gzmk-5812M
• Product Overview: Recombinant Mouse Gzmk protein(Gln44~Val227), fused with N-terminal His Tag, was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Gln44~Val227
• Form: PBS, pH7.4, containing 0.01% SKL, 5%Trehalose.
• Bio-activity: Granzyme K is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Mouse granzyme K is synthesized as a precursor (263 residues) with a signal peptide (residues 1- 21), a propeptide (residues 22-25) and a mature chain (residues 26-263). The activity of recombinant mouse Granzyme K was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl • HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μ L of various concentrations of GZMK(diluted by assay buffer) to 50 μL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMK, replace with 50 μL assay buffer.
  Incubated at 25°C for 5min, then read at a wavelength of 405 nm.
• Molecular Mass: 24.1kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Gzmk granzyme K [ Mus musculus ]
• Official Symbol: Gzmk
• Synonyms: GZMK; granzyme K
• Gene ID: 14945
• mRNA Refseq: NM_008196
• Protein Refseq: NP_032222