Recombinant Mouse Gfral protein, His-Avi-tagged

• Cat.No.: Gfral-060M
• Product Overview: Recombinant Mouse Gfral protein(Q6SJE0-1)(Gln20-Glu350), fused with N-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gln20-Glu350
• Tag: N-His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse GFRAL, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 3.0 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Gfral GDNF family receptor alpha like [ Mus musculus ]
• Official Symbol: Gfral
• Synonyms: GFRAL; GDNF family receptor alpha like; GDNF family receptor alpha-like; GDNF receptor alpha-like; Gral; AY457637
• Gene ID: 404194
• mRNA Refseq: NM_205844
• Protein Refseq: NP_995316