Recombinant Mouse Gfm1 Protein, Myc/DDK-tagged

• Cat.No.: Gfm1-3192M
• Product Overview: Purified recombinant protein of mouse full-length G elongation factor, mitochondrial 1 (Gfm1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.
• Molecular Mass: 83.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gfm1 G elongation factor, mitochondrial 1 [ Mus musculus (house mouse) ]
• Official Symbol: Gfm1
• Synonyms: GFM1; G elongation factor, mitochondrial 1; elongation factor G, mitochondrial; EF-Gmt; mEF-G 1; mitochondrial; elongation factor G1; G elongation factor 1; elongation factor G 1, mitochondrial; Gfm; AW545374; D3Wsu133e
• Gene ID: 28030
• mRNA Refseq: NM_138591
• Protein Refseq: NP_613057
• UniProt ID: Q8K0D5