Recombinant Mouse Gdf15 protein, hFc-tagged

• Cat.No.: Gdf15-01M
• Product Overview: Recombinant Mouse Gdf15 protein(Q9Z0J7)(Ser189-Ala303), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Ser189-Ala303
• Tag: N-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse GFRAL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Gdf15 growth differentiation factor 15 [ Mus musculus ]
• Official Symbol: Gdf15
• Synonyms: GDF15; growth differentiation factor 15; growth/differentiation factor 15; GDF-15; macrophage inhibiting cytokine-1; SBF; MIC-1; NAG-1
• Gene ID: 23886
• mRNA Refseq: NM_011819
• Protein Refseq: NP_035949