Recombinant Mouse Fgf7 protein(Cys32-Thr194), His-tagged

• Cat.No.: Fgf7-500M
• Product Overview: Recombinant Mouse FGF7 (NP_032034.1) (Cys32-Thr194) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Cys32-Thr194
• Form: Lyophilized from sterile 10 mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl, 140 mM NaCl, 15 % glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. The ED50 for this effect is typically 20-100 ng/mL.
• Molecular Mass: The recombinant mouse FGF7 consists of 181 amino acids and predicts a molecular mass of 21 kDa. It migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fgf7 fibroblast growth factor 7 [ Mus musculus ]
• Official Symbol: Fgf7
• Synonyms: FGF7; fibroblast growth factor 7; FGF-7; HBGF-7; Keratinocyte growth factor; heparin-binding growth factor 7; Kgf
• Gene ID: 14178
• mRNA Refseq: NM_008008
• Protein Refseq: NP_032034