Recombinant Mouse Fcgrt, His-tagged

• Cat.No.: Fcgrt-490M
• Product Overview: A DNA sequence encoding the extracellular domain (Met 1- Ser 297) of mouse FCGRT (Q61559) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse B2M (P01887 ) (Met 1- Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified.

Specification

• Description: IgG receptor FcRn large subunit p51, also known as IgG Fc fragment receptor transporter alpha chain, Neonatal Fc receptor, FCGRT and FCRN, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily. FcRn / FCGRT is a MHC class I like molecule that functions to protect IgG and albumin from catabolism. FcRn / FCGRT binds to the Fc region of monomeric immunoglobulins gamma. It mediates the uptake of IgG from milk. It may play a role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin, also known as B2M, is a secreted protein which belongs to the beta-2-microglobulin family. It contains one Ig-like C1-type (immunoglobulin-like) domain. Beta-2 microglobulin (B2M) plays a pivotal role in the biology of mammals. B2M forms the small invariable light chain subunit of class I HLA antigens on the cell membrane of all nucleated cells. During the continuous turnover of the HLA molecules. B2M is shed from the cell membrane into blood. Lymphocytes are the main source of serum free B2M. It associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules. B2M is necessary for cell surface expression of MHC class I and stability of the peptide binding groove. In fact, in the absence of B2M, very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. FcRn complex consist of two subunits: p51, and p14 which is equivalent to beta-2-microglobulin (B2M). It forms an MHC class I-like heterodimer. B2M is a component of the class I major histocompatibility complex (MHC). B2M is also an integral component of the FcRn heterodimer. Failure of passive transfer (FPT) is a condition in which neonates do not acquire protective serum levels of maternal antibodies. A principal component of antibody transport is the neonatal receptor for the Fc portion of immunoglobulin, a heterodimer of a MHC-1 alpha-chain homolog ( FcRn ) and beta-2-microglobulin ( B2M ).
• Source: Human Cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ser 22 & Ile 21
• Form: Lyophilized from sterile PBS, pH7.4.
• Molecular Mass: The recombinant heterodimer of mouse FCGRT/B2M comprises 386 (287+99) amino acids and has a calculated molecular mass of 44.2 (32.5+11.7) KDa. The apparent molecular mass of rm FCGRT/B2M heterodimer is approximately 48 & 13 KDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: hardcopy of COA with reconstitution instruction is sent along with the products.
• Protein length: 1-297;1-119 a.a.

Gene Information

• Gene Name: Fcgrt Fc receptor, IgG, alpha chain transporter [ Mus musculus (house mouse) ]
• Official Symbol: Fcgrt
• Synonyms: Fcgrt; FcRn; Fc receptor, IgG, alpha chain transporter; IgG receptor FcRn large subunit p51; neonatal Fc receptor; igG Fc fragment receptor transporter alpha chain
• Gene ID: 14132
• mRNA Refseq: NM_010189
• Protein Refseq: NP_034319
• UniProt ID: Q61559
• Chromosome Location: 7 B4; 7 29.12 cM
• Pathway: PodNet: protein-protein interactions in the podocyte; XPodNet - protein-protein interactions in the podocyte expanded by STRING
• Function: IgG binding; IgG receptor activity; beta-2-microglobulin binding