Recombinant Mouse Dohh Protein, MYC/DDK-tagged

• Cat.No.: Dohh-201M
• Product Overview: Purified recombinant protein of full-length mouse deoxyhypusine hydroxylase/monooxygenase (Dohh), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.
• Molecular Mass: 32.9 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dohh deoxyhypusine hydroxylase/monooxygenase [ Mus musculus (house mouse) ]
• Official Symbol: Dohh
• Synonyms: Dohh; deoxyhypusine hydroxylase/monooxygenase; Hlrc1; AI585913; AL022869; 1110033C18Rik; deoxyhypusine hydroxylase; DOHH; HEAT-like (PBS lyase) repeat containing 1; deoxyhypusine dioxygenase; deoxyhypusine monooxygenase; EC 1.14.99.29
• Gene ID: 102115
• mRNA Refseq: NM_133964
• Protein Refseq: NP_598725
• UniProt ID: Q99LN9