Recombinant Human DOHH Protein, GST-tagged

• Cat.No.: DOHH-2809H
• Product Overview: Human DOHH full-length ORF ( NP_112594.1, 1 a.a. - 302 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Species: Human
• Source: Wheat Germ
• Tag: GST
• Description: This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009]
• Molecular Mass: 59.3 kDa
• AA Sequence: MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEVAETCQLAVRRLEWLQQHGGEPAAGPYLSVDPAPPAEERDVGRLREALLDESRPLFERYRAMFALRNAGGEEAALALAEGLHCGSALFRHEVGYVLGQLQHEAAVPQLAAALARCTENPMVRHECAEALGAIARPACLAALQAHADDPERVVRESCEVALDMYEHETGRAFQYADGLEQLRGAPS
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: DOHH deoxyhypusine hydroxylase/monooxygenase [ Homo sapiens ]
• Official Symbol: DOHH
• Synonyms: DOHH; deoxyhypusine hydroxylase/monooxygenase; HEAT like (PBS lyase) repeat containing 1 , HLRC1; deoxyhypusine hydroxylase; MGC4293; deoxyhypusine dioxygenase; deoxyhypusine monooxygenase; HEAT-like repeat-containing protein 1; HEAT-like (PBS lyase) repeat containing 1; HLRC1; hDOHH
• Gene ID: 83475
• mRNA Refseq: NM_001145165
• Protein Refseq: NP_001138637
• MIM: 611262
• UniProt ID: Q9BU89