Recombinant Human DOHH Protein, His-tagged

• Cat.No.: DOHH-1373H
• Product Overview: Recombinant Human DOHH Protein (Met1-Leu216) with N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.4 kDa
  Accurate Molecular Mass: 28 kDa
• Protein length: Met1-Leu216
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: DOHH deoxyhypusine hydroxylase/monooxygenase [ Homo sapiens (human) ]
• Official Symbol: DOHH
• Synonyms: DOHH; deoxyhypusine hydroxylase/monooxygenase; HEAT like (PBS lyase) repeat containing 1, HLRC1; deoxyhypusine hydroxylase; MGC4293; deoxyhypusine dioxygenase; deoxyhypusine monooxygenase; HEAT-like repeat-containing protein 1; HEAT-like (PBS lyase) repeat containing 1; HLRC1; hDOHH
• Gene ID: 83475
• mRNA Refseq: NM_001145165
• Protein Refseq: NP_001138637
• MIM: 611262
• UniProt ID: Q9BU89