Recombinant Mouse Dock8 Protein, Myc/DDK-tagged

• Cat.No.: Dock8-2628M
• Product Overview: Purified recombinant protein of mouse full-length dedicator of cytokinesis 8 (Dock8), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP. During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC. Required for CD4+ T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane. Is involved in NK cell cytotoxicity controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 239.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dock8 dedicator of cytokinesis 8 [ Mus musculus (house mouse) ]
• Official Symbol: Dock8
• Synonyms: Dock8; dedicator of cytokinesis 8; AI461977; 1200017A24Rik; 5830472H07Rik; A130095G14Rik; dedicator of cytokinesis protein 8
• Gene ID: 76088
• mRNA Refseq: NM_028785
• Protein Refseq: NP_083061
• UniProt ID: Q8C147