Recombinant Mouse Dis3 Protein, Myc/DDK-tagged

• Cat.No.: Dis3-2568M
• Product Overview: Purified recombinant protein of mouse full-length DIS3 mitotic control homolog (S. cerevisiae) (cDNA clone MGC:37123 IMAGE:4952415), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 64.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dis3 DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease [ Mus musculus (house mouse) ]
• Official Symbol: Dis3
• Synonyms: DIS3; DIS3 mitotic control homolog (S. cerevisiae); exosome complex exonuclease RRP44; protein DIS3 homolog; ribosomal RNA-processing protein 44; 2810028N01Rik
• Gene ID: 72662
• mRNA Refseq: NM_028315
• Protein Refseq: NP_082591
• UniProt ID: Q9CSH3