Recombinant Mouse Dapk3 Protein, Myc/DDK-tagged

• Cat.No.: Dapk3-2453M
• Product Overview: Purified recombinant protein of mouse full-length death-associated protein kinase 3 (Dapk3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in formation of promyelocytic leukemia protein nuclear body (PML-NB). Involved in apoptosis involving PAWR which mediates cytoplasmic relocation; in vitro phosphorylates PAWR. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton such as in regulation of cell polarity and cell migration. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2; disrupts the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates STAT3 and enhances its transcriptional activity. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 51.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dapk3 death-associated protein kinase 3 [ Mus musculus (house mouse) ]
• Official Symbol: Dapk3
• Synonyms: DAPK3; death-associated protein kinase 3; ZIP kinase; ZIP-kinase; DAP kinase 3; MYPT1 kinase; DAP-like kinase; death-associated kinase 3; dlk; ZIPK
• Gene ID: 13144
• mRNA Refseq: NM_007828
• Protein Refseq: NP_031854
• UniProt ID: O54784