Recombinant Mouse Cul2 Protein, Myc/DDK-tagged

• Cat.No.: Cul2-2375M
• Product Overview: Purified recombinant protein of mouse full-length cullin 2 (cDNA clone MGC:25783 IMAGE:4018298), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
• Molecular Mass: 86.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cul2 cullin 2 [ Mus musculus (house mouse) ]
• Official Symbol: Cul2
• Synonyms: CUL2; cullin 2; cullin-2; CUL-2; AI327301; mKIAA4106; 1300003D18Rik; 4932411N15Rik; KIAA4106
• Gene ID: 71745
• mRNA Refseq: NM_029402
• Protein Refseq: NP_083678
• UniProt ID: Q9D4H8