Recombinant Mouse CD28 Protein, Fc/His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD28-640MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse CD28 precursor extracellular domain (Met 1-Lys 149) (NP_031668.3), fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus, was produced in Human Cell.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His&Fc
• Form: Lyophilized
• Molecular Mass: The recombinant mouse CD28/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 378 amino acids and has a predicted molecular mass of 43 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD28/Fc monomer is approximately 55-60 kDa due to glycosylation.
• Protein length: 378
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Cd28 CD28 antigen [ Mus musculus ]
• Official Symbol: CD28
• Gene ID: 12487
• mRNA Refseq: NM_007642
• Protein Refseq: NP_031668