Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein, Fc-tagged (HPLC-verified)

• Cat.No.: CD28-562H
• Product Overview: Recombinant Human/Cynomolgus/Rhesus macaque CD28 (Asn19-Pro152) protein (HPLC-verified) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
• Source: HEK293
• Species: Human/Cynomolgus/Rhesus macaque
• Tag: Fc
• Predicted N Terminal: Asn19
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 41.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Asn19-Pro152
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD28
• Official Symbol: CD28
• Synonyms: Tp44
• Gene ID: 940
• mRNA Refseq: NM_006139.4
• Protein Refseq: NP_006130.1
• UniProt ID: P10747