Recombinant Mouse CCNE1, His-GST tagged

• Cat.No.: CCNE1-416M
• Product Overview: Recombinant Mouse CCNE1 (AAH62152.1) (Met1-Glu408), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: Non
• Protein Length: 1-408 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 50mM Tris, 100mM Nacl, 3mM DTT, 0.5mM GSH, 10% gly, pH 8.0.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse CCNE1/GST chimera consists of 645 amino acids and has a calculated molecular mass of 74.8 kDa. The recombinant protein migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Ccne1 cyclin E1 [ Mus musculus ]
• Official Symbol: CCNE1
• Gene ID: 12447
• mRNA Refseq: NM_007633
• Protein Refseq: NP_031659