Recombinant Mouse CCNE1 protein(Met1-Glu408), His&GST-tagged

• Cat.No.: CCNE1-3244M
• Product Overview: Recombinant Mouse CCNE1 (AAH62152.1) (Met1-Glu408) was expressed in Insect Cells with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Met1-Glu408
• Form: Lyophilized from sterile 50mM Tris, 100mM Nacl, 3mM DTT, 0.5mM GSH, 10% gly, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse CCNE1/GST chimera consists of 645 amino acids and has a calculated molecular mass of 74.8 kDa. The recombinant protein migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ccne1 cyclin E1 [ Mus musculus ]
• Official Symbol: CCNE1
• Synonyms: CCNE1; cyclin E1; G1/S-specific cyclin-E1; CycE1; AW538188
• Gene ID: 12447
• mRNA Refseq: NM_007633
• Protein Refseq: NP_031659