Recombinant Mouse C3 Protein, His/GST-tagged

• Cat.No.: C3-559M
• Product Overview: Recombinant Mouse C3(Ser671~Arg748) fused with His/GST tag at N-terminal was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: GST&His
• Protein Length: Ser671~Arg748
• Description: This gene encodes complement protein C3 which plays a central role in the classical, alternative and lectin activation pathways of the complement system. The encoded preproprotein undergoes a multi-step processing to generate various functional peptides. Mice deficient in the encoded protein fail to clear bacteria from the blood stream upon infection, display diminished airway hyperresponsiveness and lung eosinophilia upon allergen-induced pulmonary allergy, and develop severe lung injury after deposition of IgG immune complexes. Deficiency of the homolog of the encoded protein in humans was found to be associated with increased susceptibility to infections, age-related macular degeneration, and atypical hemolytic uremic syndrome.
• Form: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 41.2kDa
• Identity: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: C3 complement component 3 [ Mus musculus ]
• Official Symbol: C3
• Synonyms: C3; complement component 3; complement C3; complement factor 3; complement component 3d; acylation stimulating protein; ASP; Plp; HSE-MSF; AI255234
• Gene ID: 12266
• mRNA Refseq: NM_009778
• Protein Refseq: NP_033908
• UniProt ID: P01027