Active Native Botulinum C3 Enzyme

• Cat.No.: C3-147C
• Product Overview: This product was highly purified from the supernatant of the culture of Clostridium botulinum type C by cation exchange, hydroxyapatite and gel filtration columns. This toxin ADP–ribosylates Rho and Rac, which are low-molecular weight GTP-binding proteins of animal cells, and interrupts the downstream signal transduction pathways. The molecular weight is 24kDa.

Specification

• Species: Clostridium Botulinum
• Source: Clostridium botulinum D bacteriophage
• Tag: Non
• Form: 1.33 mg/ml in 5 mM sodium phosphate buffer (pH 6.0), 50% glycerol
• Bio-activity: Stimulation of human keratinocytes in culture was observed at 50 ng/ml.
• Purity: More than 90 % purity by SDS-PAGE (CBB staining)
• Applications: 1) For the studies on the Rho-dependent signaling pathways in animal cells in vivo and in vitro. 2) ADP-ribosylation of small GTP proteins, such as Rho and Rac proteins.
• Storage: Store at -20℃ (long period; -70℃)