Recombinant Mouse ASGPR1 Protein (Ser60-Asn284), His-tagged, Biotinylated

• Cat.No.: ASGPR1-1069M
• Product Overview: Recombinant Mouse ASGPR1 Protein (NP_033844.1) (Ser60-Asn284) was produced by HEK293 Cells expression system. This protein was expressed with a N-terminal polyhistidine tag. The purified protein was biotinylated in vitro.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• ProteinLength: Ser60-Asn284
• Description: The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development.
• Predicted N Terminal: His
• Form: Lyophilized from sterile PBS, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse ASGR1 consists of 241 amino acids and with a predicted molecular mass of 28 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Asgr1 asialoglycoprotein receptor 1 [ Mus musculus (house mouse) ]
• Official Symbol: ASGPR1
• Synonyms: Asgr; HL-1; ASGPR1; Asgr-1
• Gene ID: 11889
• mRNA Refseq: NM_009714
• Protein Refseq: NP_033844
• UniProt ID: P34927