Recombinant Human MMAB 293 Cell Lysate
Cat.No. : | MMAB-4285HCL |
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Species : | Human |
Source : | HEK293 |
Tag : | Non |
Description : | Antigen standard for methylmalonic aciduria (cobalamin deficiency) cblB type (MMAB), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name | MMAB methylmalonic aciduria (cobalamin deficiency) cblB type [ Homo sapiens ] |
Official Symbol | MMAB |
Synonyms | MMAB; methylmalonic aciduria (cobalamin deficiency) cblB type; methylmalonic aciduria (cobalamin deficiency) type B; cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial; ATP:cob(I)alamin adenosyltransferase; cblB; ATP:corrinoid adenosyltransferase; methylmalonic aciduria type B protein; aquocob(I)alamin vitamin B12s adenosyltransferase; ATR; cob; MGC20496; |
Gene ID | 326625 |
mRNA Refseq | NM_052845 |
Protein Refseq | NP_443077 |
MIM | 607568 |
UniProt ID | Q96EY8 |
Chromosome Location | 12q24 |
Pathway | Metabolic pathways, organism-specific biosystem; Porphyrin and chlorophyll metabolism, organism-specific biosystem; Porphyrin and chlorophyll metabolism, conserved biosystem; |
Function | ATP binding; cob(I)yrinic acid a,c-diamide adenosyltransferase activity; nucleotide binding; transferase activity; |
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Not For Human Consumption!
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