Recombinant Mouse ARSA protein(Met1-Ser506), His-tagged

• Cat.No.: Arsa-2334M
• Product Overview: Recombinant Mouse ARSA (NP_033843.2)(Met 1-Ser 506) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-506 a.a.
• Form: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave p-Nitrocatechol Sulfate (PNCS). The specific activity is >100 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse ARSA consists of 500 amino acids and has a calculated molecular mass of 53.5 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 60 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Arsa arylsulfatase A [ Mus musculus ]
• Official Symbol: ARSA
• Synonyms: ARSA; arylsulfatase A; cerebroside-sulfatase; ASA; As2; AS-A; As-2; TISP73; AW212749
• Gene ID: 11883
• mRNA Refseq: NM_009713
• Protein Refseq: NP_033843