Active Recombinant Human ARSA, His-tagged

• Cat.No.: ARSA-306H
• Product Overview: Recombinant Human ARSA (Accession # AAH14210) Arg19-Ala507, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Arg19
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is >25 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human ARSA, His-tagged has a calculated MW of 53 kDa. In SDS-PAGE migrates as 63 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Protein length: 19-507 a.a.

Gene Information

• Gene Name: ARSA arylsulfatase A [ Homo sapiens ]
• Official Symbol: ARSA
• Synonyms: ARSA; arylsulfatase A; metachromatic leucodystrophy; ASA; cerebroside-sulfatase; MLD
• Gene ID: 410
• mRNA Refseq: NM_000487
• Protein Refseq: NP_000478
• MIM: 607574
• UniProt ID: P15289
• Chromosome Location: 22q13.31-qter