Active Recombinant Human ARSA Protein (21-509), C-His-tagged

• Cat.No.: ARSA-14H
• Product Overview: ARSA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 498 amino acids (21-509a.a.) and having a molecular mass of 53.0kDa. (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). ARSA is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 21-509
• Description: The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.
• Bio-activity: Specific activity is > 2,500 pmol/min/μg, and defined as the amount of enzyme that hydrolyze 4-Nitrocatechol at pH 5.0 at 37 centigrade.
• Molecular Mass: 53.0 kDa
• AA Sequence: ADPRPPNIVLIFADDLGYGDLGCYGHPSSTTPNLDQLAAGGLRFTDFYVPVSLCTPSRAALLTGRLPVRMGMYPGVLVPSSRGGLPLEEVTVAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQGFHRFLGIPYSHDQGPCQNLTCFPPATPCDGGCDQGLVPIPLLANLSVEAQPPWLPGLEARYMAFAHDLMADAQRQDRPFFLYYASHHTHYPQFSGQSFAERSGRGPFGDSLMELDAAVGTLMTAIGDLGLLEETLVIFTADNGPETMRMSRGGCSGLLRCGKGTTYEGGVREPALAFWPGHIAPGVTHELASSLDLLPTLAALAGAPLPNVTLDGFDLSPLLLGTGKSPRQSLFFYPSYPDEVRGVFAVRTGKYKAHFFTQGSAHSDTTADPACHASSSLTAHEPPLLYDLSKDPGENYNLLGGVAGATPEVLQALKQLQLLKAQLDAAVTFGPSQVARGEDPALQICCHPGCTPRPACCHCPDPHAHHHHHH.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Notes: Our products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
• Storage: Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
• Concentration: 0.25 mg/mL
• Storage Buffer: Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

Gene Information

• Gene Name: ARSA arylsulfatase A [ Homo sapiens (human) ]
• Official Symbol: ARSA
• Synonyms: ARSA; arylsulfatase A; ASA; MLD; arylsulfatase A; cerebroside-sulfatase; epididymis secretory sperm binding protein; EC 3.1.6.8
• Gene ID: 410
• mRNA Refseq: NM_000487
• Protein Refseq: NP_000478
• MIM: 607574
• UniProt ID: P15289