Recombinant Mouse APOE Protein, His-tagged

• Cat.No.: APOE-58M
• Product Overview: Recombinant Mouse Apolipoprotein E is produced by our Mammalian expression system and the target gene encoding Glu19-Gln311 is expressed with a 6His tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 19-311 a.a.
• Description: Apolipoprotein E (ApoE) is a glycoprotein that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. ApoE can be synthesized by the liver and intestinally synthesized chylomicrons. ApoE mediates high-affinity binding of chylomicrons and very low density lipoprotein (VLDL) particles to the LDL receptor, allowing for specific uptake of these particles by the liver, preventing the accumulation of cholesterol-rich particles in the plasma. ApoE plays an essential role in the formation of plasma lipoproteins and is involved in their production, conversion and clearance. ApoE is also a constituent of a subclass of high density of lipoproteins (HDL) involved in cholesterol transport. More recently, ApoE has been studied for its role in several biological processes not directly related to lipoprotein transport, including Alzheimer's disease, immunoregulation, and cognition. Though the exact mechanisms remain to be clarified, isoform 4 of APOE, has been associated with increased calcium ion levels and apoptosis following mechanical injury.
• Form: Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 0.1%Tween 80, 10% trehalose, pH 7.4.
• Molecular Mass: Predicted Molecular Weight: 34.8kDa
  Apparent Molecular Weight: 35kDa, reducing conditions.
• AA Sequence: Glu19-Gln311
• Endotoxin: Less than 0.1 EU/ug.
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days. Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: Apoe apolipoprotein E [ Mus musculus (house mouse) ]
• Official Symbol: APOE
• Synonyms: Apolipoprotein E; Apo-E;APOE
• Gene ID: 11816
• mRNA Refseq: NM_009696.4
• Protein Refseq: NP_033826.2
• UniProt ID: P08226