Recombinant Monkeypox virus A30 protein, His-tagged

• Cat.No.: A30-1545M
• Product Overview: Recombinant Monkeypox virus A30 protein(Q8V4U9)(Gln22-Leu146), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: MPX
• Source: N-His
• ProteinLength: Gln22-Leu146
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Molecular Mass: The protein has a predicted MW of 15.24 kDa. Due to glycosylation, the protein migrates to 30-55 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.