Recombinant Human PSMC3IP 293 Cell Lysate

• Cat.No.: PSMC3IP-2762HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for PSMC3 interacting protein (PSMC3IP), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PSMC3IP PSMC3 interacting protein [ Homo sapiens ]
• Official Symbol: PSMC3IP
• Synonyms: PSMC3IP; PSMC3 interacting protein; homologous-pairing protein 2 homolog; GT198; HUMGT198A; TBP 1 interacting protein; TBPIP; DBD-interacting; TBP-1-interacting protein; nuclear receptor coactivator GT198; tat-binding protein 1-interacting protein; proteasome 26S ATPase subunit 3-interacting protein; HOP2; ODG3
• Gene ID: 29893
• mRNA Refseq: NM_001256014
• Protein Refseq: NP_001242943
• MIM: 608665
• UniProt ID: Q9P2W1
• Chromosome Location: 17q21.2
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Meiosis, organism-specific biosystem; Meiotic Recombination, organism-specific biosystem
• Function: DBD domain binding; DNA binding; androgen receptor binding; estrogen receptor binding; glucocorticoid receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; protein homodimerization activity; thyroid hormone receptor binding