Recombinant Human WNT1 Inducible Signaling Pathway Protein 2

• Cat.No.: WISP2-932H
• Product Overview: Recombinant human CTGFL/WISP-2 produced inE.coliis a 24.8 kDa protein of 228 amino acid residues.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: CTGFL/WISP-2 is a 24.3 kDa protein that belongs to the CCN family of cysteine rich regulatory proteins. Members of this family stimulate mitosis, adhesion, apoptosis, extracellular matrix production, growth arrest, and migration of multiple cell types. The protein is expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart. In addition to promoting adhesion of osteoblasts, CTGFL/WISP-2 inhibits osteocalcin production, as well as binding of fibrinogen to integrin receptors.
• Purity: > 95% by SDS-PAGE and HPLC analysis.
• Endotoxin level: < 0.1 ng per μg of CTGFL/WISP-2
• Stabilizer: None.
• Formulation: Lyophilized.
• Biologically Activity: The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is between 10-20 ng/ml in the presence of 15 ng/ml of human IGF-II.
• Reconstitution: The human CTGFL/WISP-2 should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C.
• Stability: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted human CTGFL/WISP-2 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Publications:
  Non-canonical HIF-2a function drives autonomous breast cancer cell growth via an AREG–EGFR/ErbB4 autocrine loop (2012)

Gene Information

• Gene Name: WISP2 WNT1 inducible signaling pathway protein 2 [ Homo sapiens ]
• Synonyms: WISP2; WNT1 inducible signaling pathway protein 2; CCN5; CT58; CTGF-L; OTTHUMP00000063227; wnt-1 signaling pathway protein 2; connective tissue growth factor-like protein; WNT1-inducible-signaling pathway protein 2; WISP-2; Connective tissue growth factor-related protein 58; OTTHUMP00000031770
• Gene ID: 8839
• mRNA Refseq: NM_003881
• Protein Refseq: NP_003872
• MIM: 603399
• UniProt ID: O76076
• Chromosome Location: 20q13.12
• Function: insulin-like growth factor binding; protein binding