Recombinant Human WFS1 protein, His-tagged

• Cat.No.: WFS1-3617H
• Product Overview: Recombinant Human WFS1 protein(1-95 aa), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E. coli
• Tag: His
• Protein Length: 1-95 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole.
• AASequence: MDSNTAPLGPSCPQPPPAPQPQARSRLNATASLEQERSERPRAPGPQAGPGPGVRDAAAPAEPQAQHTRSRERADGTGPTKGDMEIPFEEVLERA
• Purity: 90%, by SDS-PAGE.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: WFS1 Wolfram syndrome 1 (wolframin) [ Homo sapiens ]
• Official Symbol: WFS1
• Synonyms: WFS1; Wolfram syndrome 1 (wolframin); DFNA6, DFNA14, DFNA38; wolframin; DIDMOAD; WFS; WFRS; WFSL; FLJ51211
• mRNA Refseq: NM_001145853
• Protein Refseq: NP_001139325
• MIM: 606201
• UniProt ID: O76024
• Gene ID: 7466