Recombinant Human WEE1 Protein (M291-K575), Tag Free

• Cat.No.: WEE1-0059H
• Product Overview: Recombinant Human (G)-Wee1(M291-K575) Protein was expressed in Insect cell.

Specification

• Description: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
• Source: Insect cell
• Species: Human
• Tag: Non
• Form: Liquid
• Protein length: M291-K575
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: WEE1 WEE1 homolog (S. pombe) [ Homo sapiens (human) ]
• Official Symbol: WEE1
• Synonyms: WEE1; WEE1 homolog (S. pombe); wee1+ (S. pombe) homolog; wee1-like protein kinase; wee1A kinase; WEE1+ homolog; WEE1A; WEE1hu; FLJ16446; DKFZp686I18166
• Gene ID: 7465
• mRNA Refseq: NM_001143976
• Protein Refseq: NP_001137448
• MIM: 193525
• UniProt ID: P30291

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Q&As

How does WEE1 contribute to DNA damage response?

It plays a role in DNA damage response by delaying cell cycle progression to allow DNA repair.

What is the impact of altered WEE1 activity on cancer development?

Altered WEE1 activity is associated with the progression of various cancers.

What potential therapeutic applications arise from targeting WEE1 in cancer treatment?

Targeting WEE1 in cancer therapy could exploit its role in cell cycle regulation to inhibit tumor growth.

How do genetic variations in WEE1 affect cell division?

Genetic mutations in WEE1 can lead to uncontrolled cell division and genomic instability.

What role does WEE1 play in checkpoint regulation?

WEE1 is critical in checkpoint regulation, ensuring cells do not divide with damaged DNA.

How does WEE1 interact with other cell cycle proteins?

WEE1 works in conjunction with other cell cycle proteins to regulate the timing of cell division.

What is the primary role of WEE1 in cell cycle control?

WEE1 is a kinase that inhibits cell cycle progression, particularly at the G2/M checkpoint.